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<p><b>OBJECTIVE</b>To investigate the effect of semen-derived enhancer of virus infection (SEVI) on the infection of transmitted/founder (TF) HIV-1 and its matched chronic control (CC) viruses and the antagonism of ADS-J1 on SEVI-mediated enhancement of TF and CC virus infection in vitro.</p><p><b>METHODS</b>PAPself-assembling into SEVI amyloid fibrils was validated by ThT assay. We generated the virus stocks of TF and CC virus pair. TZM-bl cells were infected with the mixture of SEVI and TF or CC viruses for 72 h. Luciferase activity was used to observe the enhancement of SEVI. SEVI was treated with different concentrations of ADS-J1 and incubated with TF or CC viruses. TZM-bl cells were then infected with the mixture and luciferase activity was detected 72 h after infection to analyze the antagonism of ADS-J1 on the enhancing effect of SEVI. ADS-J1 was also incubated with TF and CC viruses directly and TZM-bl cells were infected for 72 h to evaluate the antiviral effect using luciferase assay. SEVI was treated with ADS-J1 and Zeta potential was determined to explore the antagonistic mechanism of ADS-J1.</p><p><b>RESULTS</b>ThT assay showed that PAPwas capable of self-assembly into SEVI amyloid fibrils. SEVI significantly accelerated TF and CC viruses infection (P<0.05), and ADS-J1 not only significantly antagonized the enhancement of SEVI (P<0.05) but also directly inhibited the infection of TF and CC viruses (P<0.05). ADS-J1 neutralized the positive charge of SEVI in a dose-dependent manner.</p><p><b>CONCLUSIONS</b>SEVI promotes the infection of TF and CC strains, and ADS-J1 antagonizes SEVI-mediated enhancement of TF and CC viruses by neutralizing the positive charge of SEVI.</p>
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Objective To explore the efficacy and safety of Shexiang Baoxin Pill combined with isosorbidedinitrate in the treatment of unstable angina pectoris.Methods 80 cases of UAP patients from Jun.2014 to Jun.2016 were given basic treatment plan,and 40 cases were randomly selected and treated with isosorbidedinitrate as the control group,and the other 40 cases were treated with Shexiang Baoxin Pill combined with isosorbidedinitrate.The incidence of angina pectoris and nitroglycerin dosage,angina pectoris curative effect,electrocardiogram (ECG) curative effect and adverse drug reactions were compared between the two groups.Results Before treatment,daily anginal attacks and nitroglycerin dosage of two groups had no significant difference;after treatment,daily anginal attacks and nitroglycerin dosage between two groups were obviously decreased,the differences of same group before and after treatment was statistically significant (P ＜ 0.05);and the treatment group was significantly less than the control group (P ＜ 0.05).The total effective rate of angina pectoris in the treatment group was higher than that in the control group,the difference was statistically significant (P ＜ 0.05).The total effective rate of ECG in the treatment group was higher than that in the control group,the difference was statistically significant (P ＜ 0.05).Conclusions Shexiang Baoxin Pill combined with isosorbidedinitrate in the treatment of UAP can significantly improve the efficacy,and it's adverse reaction is less.
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<p><b>OBJECTIVE</b>To study the clinical efficacy of TH glue (cyanoacrylate) obliteration of esophageal varices and partial spleen embolization (PSE) in the treatment of esophageal varices bleeding.</p><p><b>METHODS</b>TH glue was injected into the gastroesophageal varices and their feeder veins in 84 patients. According to the size of the varices and their blood flow, the TH glue was injected alone or after a steel coil was placed at the ostium of the feeder vein. Sometimes absolute alcohol was also injected into the varices. PSE was performed after the TH glue obliteration in all patients.</p><p><b>RESULTS</b>(1) The TH glue obliteration was performed on 81 patients with a success rate of 96.4%. Three patients died from the procedure. (2) The left gastric vein coronary, gastric varices, pericardial varices, and lower esophageal veins were obliterated in 38 patients (group 1). Left gastric vein, gastric varices, and pericardial veins were obliterated in 31 patients (group 2). Only the main gastric coronary vein was obliterated in 9 patients (group 3). The disappearance rate of the esophageal varices was 71.1% (27/38), 35.5% (11/31) and 0% (0/9) in the three groups. (3) During a follow-up of 6-49 months, rebleeding occurred in 13 of all 78 (16.7%) patients, and it was 7.9% (3/38), 12.9% (4/31) and 66.7% (6/9) respectively in the three groups.</p><p><b>CONCLUSION</b>Our results suggest percutaneous transhepatic TH glue obliteration of esophageal varices and PSE are safe and effective in treating gastroesophageal varicose bleeding.</p>
Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Child , Female , Humans , Male , Middle Aged , Young Adult , Combined Modality Therapy , Cyanoacrylates , Therapeutic Uses , Embolization, Therapeutic , Methods , Esophageal and Gastric Varices , Therapeutics , Spleen , Tissue Adhesives , Therapeutic UsesABSTRACT
0.05).The variced recurrence rate in 1- and 2-year were 12% (3/25) and 20% (5/25) in TH glue group,and 39.1% (9/23) and 86.9%(20/23) in control group (P